Enzymes and Inhibitors
New England Biolabs, Inc. BcgI – 250 units
A restriction endonuclease that recognizes the sequence _NN^NNNNNNNNNNCGANNNNNNTGCNNNNNNNNNN_NN^.
New England Biolabs, Inc. NgoMIV – 1000 units
A restriction endonuclease that recognizes the sequence G^CCGG_C.
New England Biolabs, Inc. BmrI – 100 units
A restriction endonuclease that recognizes the sequence ACTGGGNNNN_N^.
Ubpbio SUC-LLVY-AMC, 5MG
Fluorescent Peptides/Protein Substrates – SUC-LLVY-AMC (UBPBio catalog# G1100, G1101) – Suc-LLVY-AMC is a fluorogenic substrate for the chymotrypsin – like activity of the 20S and 26S proteasomes. Working concentrations of this substrate is 50-200 µM. The released AMC can be detected by a fluorimeter or plate reader at excitation/emission wavelengths of 380 nm/460 nm, respectively. When used to determine proteasome activity in cell lysates, cell lysates that are pre-treated with a proteasome inhibitor such as MG132, PS341 or epoxomicin can be used to determine the fluorescence contributed by other cellular proteases that cleave this substrate. Readings from proteasome inhibitor-treated lysates should be subtracted.
Prolume Ltd Coelenterazine h | 50909-86-9 | MFCD00798394 | 10 mg
Coelenterazine h | Mol Wt: 407.5 | 50909-86-9 | MFCD00798394 | 10 mg
New England Biolabs, Inc. TaqI-v2 – 4000 units
A restriction endonuclease that recognizes the sequence T^CG_A.
Worthington Biochemical Corporation COLLAGNASE 250MG (5 PK 50MG)
Collagenase, Type 3, Filtered, which is filtered through a 0.22 Micron membrane and lyophilized in vials to contain > /=50mg/VL, Store at 2 to 8degreeC, Pack Size: 5 x 50mg, Source: Clostridium histolyticum, Minimum Activity: > /=100U/mg dr
Bioworld TMB Stop Reagent, 450 nm, 100 mL
TMB Stop ReagentStop reagent is 0.16M sulfuric acid used to stop the ELISA substrate 3,3’,5,5’ - tetramethylbenzidine (TMB) reaction. Horseradish peroxidase (HRP) enzyme conjugates react withTMB and peroxide to produce a blue byproduct having maximum absorbance at 605nm. Adding Stop reagent changes it to yellow color with absorption maximum at 450 nm and stabilizes the color development.Quantity: 100ml
Worthington Biochemical Corporation Deoxyribonuclease I, 25mg
Partially purified. A lyophilized powder. Store at 2-8°C. Bovine pancreatic deoxyribonuclease is an endonuclease which splits phosphodiester linkages, preferentially adjacent to a pyrimidine nucleotide yielding polynucleotides with free hydroxyl group at the 3' position and phosphate group at the 5' position. The average chain length of a limit digest is a tetranucleotide.
Worthington Biochemical Corporation PEROXIDASE HORSERADISH 100MG
A soluble, dialyzed, lyophilized powder. RZ > or = 1.0.
New England Biolabs, Inc. dam Methyltransferase – 500 units
dam Methyltransferase modifies the adenine residue (N6) of the sequence GATC.
New England Biolabs, Inc. DNase I-XT-1000 units
An engineered variant of DNase I DNase I-XT is a salt-tolerant DNA endonuclease that nonspecifically cleaves DNA to release di- tri- and oligonucleotide products with 5 prime phosphorylated and 3 prime hydroxylated ends. DNase I-XT acts on single- and double-stranded DNA chromatin and the DNA strand of RNADNA hybrids. While DNase I (M0303) is inhibited by salt concentrations greater than 50 mM DNase I-XT exhibits optimal activity between 50-100 mM salt and retains 65% and about 40% activity in 200 and 300 mM salt respectively. This increased salt tolerance makes DNase I-XT the preferred enzyme for DNA template removal from an in vitro transcription (IVT) reaction. Importantly DNase I-XT is RNase-free allowing for the complete removal of DNA from RNA preparations while maintaining RNA integrity.
New England Biolabs, Inc. Lambda Exonuclease – 5000 units
Lambda Exonuclease is a processive enzyme that catalyzes 5' to 3' mononucleotide removal from dsDNA with a preferred substrate of a 5' phosphate. Ideal for conversion of linear double-stranded DNA to single-stranded DNA via preferred activity on 5'-phosphorylated ends.
New England Biolabs, Inc. Exonuclease I (E.coli) – 15000 units
Exonuclease I is a DNA-specific endonuclease that catalyzes the removal of nucleotides from linear single-stranded DNA in the 3' to 5' direction. Note: See also Exo-CIP Rapid PCR Cleanup Kit.
New England Biolabs, Inc. AvaII – 2000 units
A restriction endonuclease that recognizes the sequence G^GWC_C.